using CV of a reference to predict the rate of false positives and false negatives

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Imefungwa

an assay has a CV of 15% for a standard reference, ie, the same substance tested in the same way in the same lab; all known variables have been controlled and yet there is still a CV of 15%. Supposing the assay variability applies equally to a test substance. Is it possible to estimate the chance of a false positive and a false negative from the variability for the reference and identical variability for the test substance? the reference is used to normalize results across different studies. How should I describe the risk posed by normalization to a reference that is so variable?

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